AUTHOR=Cui Hu , Wang Lixia , Zhang Jian 

TITLE=Synergistic influence on microbial communities ascribed to copper and tetracycline during aerobic composting: Insights into bacterial and fungal structures

JOURNAL=Frontiers in Ecology and Evolution

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2022.1019494

DOI=10.3389/fevo.2022.1019494

ISSN=2296-701X

ABSTRACT=There are considerable discussions aimed at ascertaining microbial communities and functions during the composting process. However, microbial succession under copper (Cu) and tetracycline (TCH)-stressed conditions has received less attention. Thus, this work analyzed the bacterial and fungal structures with high-throughput sequencing in Cu/TCH-amended composting (Cu: 0, 100 and 500 mg/kg; TCH: 0, 50 and 300 mg/kg), and the dominating controls on microbial diversity were identified using redundancy analysis (RDA) and structure equation models (SEMs). Low-concentration Cu increased the peak temperature (57℃) at the thermophilic phase. Composting phase-derived changes in bacterial and fungal communities were significant, while Cu and TCH showed a remarkable influence on fungi, but not to bacteria. Cu and TCH inhibited Firmicutes activity, while promoted Actinobacteriota growth. Low-concentration Cu and TCH had a negative effect on Basidiomycota at the thermophilic phase, and a positive influence on Chytridiomycota at the mature phase. TOC and TN were primary controls on the changes in microbial communities. NH4+-N and NO3--N were more beneficial to fungi with the contribution proportion of 42.13% and 16.85%, respectively. These findings could provide theoretical guidance for the directional research on the microbial inoculants.