AUTHOR=González-Rabanal Borja , Marín-Arroyo Ana B. , Carmona-Ballestero Eduardo , Cuenca-Solana David , Gutiérrez-Zugasti Igor , Martín-Merino Miguel Ángel , Ortega-Martínez Ana Isabel , Straus Lawrence G. , Vega-Maeso Cristina , González Morales Manuel R. , Cristiani Emanuela TITLE=New insights into prehistoric agriculture of northern Iberia from the analysis of starch grains embedded in dental calculus JOURNAL=Frontiers in Ecology and Evolution VOLUME=Volume 13 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2025.1644052 DOI=10.3389/fevo.2025.1644052 ISSN=2296-701X ABSTRACT=IntroductionResearch on the origin and spread of agriculture in northern Atlantic Iberia remains partially limited because archaeobotanical data are scarce due to old excavations or preservation biases.MethodsIn this paper, we present starch grain analyses of dental calculus of 18 individuals from 10 sites dated to the 4th/2nd millennium cal BCResultsThis research supports the development of extensive and stable agriculture, based on the cultivation of wheat and barley species, from the Neolithic to the Bronze Age, when millets were introduced, marking the primary shift of the Cantabrian farming economy and revealing a more diversified and mixed agriculture thereafter. In parallel, legumes were less cultivated since the Neolithic and probably used as a secondary plant resource by the Cantabrian communities.DiscussionOur results have also allowed us to corroborate previous regional archaeobotanical and isotopic data and enhance the archaeological evidence of plant consumption during Late Prehistory, establishing a diachronic multiapproach to the development of agricultural practices in this area and providing a methodological framework for future studies.