AUTHOR=Maben Zachary J. , Malik Sundeep , Jiang Liyi H. , Hinkle Patricia M. TITLE=Dual Topology of the Melanocortin-2 Receptor Accessory Protein Is Stable JOURNAL=Frontiers in Endocrinology VOLUME=Volume 7 - 2016 YEAR=2016 URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2016.00096 DOI=10.3389/fendo.2016.00096 ISSN=1664-2392 ABSTRACT=
Melanocortin 2 receptor accessory protein (MRAP) facilitates trafficking of melanocortin 2 (MC2) receptors and is essential for ACTH binding and signaling. MRAP is a single transmembrane domain protein that forms antiparallel homodimers. These studies ask when MRAP first acquires this dual topology, whether MRAP architecture is static or stable, and whether the accessory protein undergoes rapid turnover. To answer these questions, we developed an approach that capitalizes on the specificity of bacterial biotin ligase, which adds biotin to lysine in a short acceptor peptide sequence; the distinct mobility of MRAP protomers of opposite orientations based on their