AUTHOR=Jiang Mengxue , Kuang Zhijian , He Yaohui , Cao Yin , Yu Tingyan , Cheng Jidong , Liu Wen , Wang Wei 

TITLE=SNAPIN Regulates Cell Cycle Progression to Promote Pancreatic β Cell Growth

JOURNAL=Frontiers in Endocrinology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2021.624309

DOI=10.3389/fendo.2021.624309

ISSN=1664-2392

ABSTRACT=In diabetes mellitus, death of β cell in the pancreas occurs throughout the development of the disease, with loss of insulin production. The maintenance of β cell number is essential to maintaining normoglycemia. SNAPIN has been found to regulate insulin secretion, but whether SNAPIN induces β cell proliferation remains to be elucidated. This study aimed to explore the physiological roles of SNAPIN in β cell proliferation. SNAPIN expression increased with the age of mice and SNAPIN is down-expressed in diabetes. KEGG pathway and GO analysis showed that SNAPIN and its interacting proteins enrich the cell cycle. Β cell cycle was arrested in the S phase after SNAPIN knockdown, and cell proliferation was inhibited after SNAPIN knockdown. The expression of proteins CDK2, CDK4 and CCND1 expression in the S phase of the cell cycle were reduced after SNAPIN knockdown. And the expression of proteins CDK2, CDK4 and CCND1 expression in the S phase of the cell cycle were increased after overexpression of SNAPIN. In addition, insulin protein/mRNA levels also increased or decreased after SNAPIN knockdown or overexpression, respectively. Conclusions: Our data indicate that SNAPIN mediates β cells proliferation and insulin secretion, and supply the evidence for the SNAPIN/analog being a pharmacotherapeutic target for diabetes mellitus.