AUTHOR=He Rongjun , Mowery Stephanie A. , Chabenne Joseph , Finan Brian , Mayer John P. , DiMarchi Richard D. TITLE=A Facile Procedure for One-Pot Stable Conjugation of Two Proglucagon Cysteine-Containing Peptide Analogs JOURNAL=Frontiers in Endocrinology VOLUME=Volume 12 - 2021 YEAR=2021 URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2021.693958 DOI=10.3389/fendo.2021.693958 ISSN=1664-2392 ABSTRACT=An efficient, one pot site-specific conjugation of two cysteine-containing peptides was achieved by use of a maleimide/iodoacetyl bivalent linker. Following the initial ligation step, the linker was converted from an unstable thiosuccinimide to a stable thioether by mild aqueous hydrolysis. This method enables efficient, straightforward peptide conjugation for biochemical and medicinal applications.