AUTHOR=Guan Kaiyu , Shan Chunyan , Guo Anqi , Gao Xiang , Li Xi 

TITLE=Ghrelin regulates hyperactivity-like behaviors via growth hormone signaling pathway in zebrafish (Danio rerio)

JOURNAL=Frontiers in Endocrinology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2023.1163263

DOI=10.3389/fendo.2023.1163263

ISSN=1664-2392

ABSTRACT=Ghrelin was originally identified as the endogenous ligand for the growth hormone secretagogue receptor and partially acts by stimulating growth hormone (GH) release. Our previous studies have identified GHRELIN is a new susceptibility gene for human ADHD, and ghrelin-depleted zebrafish (Danio rerio) display ADHD-like behaviors. Here, we performed RNA-sequencing analysis using adult ghrelinΔ/Δ zebrafish brain to investigate the underlying molecular change, and found that transcriptional expression of gh1 mRNA and it signaling pathway-related genes were significantly reduced, quantitative PCR reproduced these results in in ghrelinΔ/Δzebrafish larvae and the brain of adult ghrelinΔ/Δzebrafish. In addition, injection of recombinant human growth hormone (rhGH) can partially save the hyperactivity of ghrelinΔ/Δ zebrafish. Our results indicated that ghrelin regulate hyperactivity-like behaviors by mediating the GH signal pathway in zebrafish.