AUTHOR=Cassano Velia , Pelaia Corrado , Armentaro Giuseppe , Miceli Sofia , Tallarico Valeria , Perini Daniele Dallimonti , Fiorentino Vanessa T. , Imbalzano Egidio , Maio Raffaele , Succurro Elena , Hribal Marta L. , Andreozzi Francesco , Sesti Giorgio , Sciacqua Angela 

TITLE=New potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance status

JOURNAL=Frontiers in Endocrinology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2023.1206336

DOI=10.3389/fendo.2023.1206336

ISSN=1664-2392

ABSTRACT=The purpose of the present study was to investigate the role of oxidative stress, platelets activation and endocan levels in renal dysfunction, in normal glucose tolerance patients (NGT) with 1-hour plasma glucose values ≥ 155 mg/dL (NGT≥155), compared to NGT<155, impaired glucose tolerance (IGT) and type 2 diabetes mellitus (T2DM) newly diagnosed subjects. We enlisted 233 patients subjected to OGTT.The serum levels of platelets activation (Glycoprotein-VI and sP-selectin) oxidative stress biomarkers (8-isoprostane and Nox-2), and endocan were evaluated with ELISA test.Results: From NGT<155 to T2DM group, there was a statistically significant increase in 8-isoprostane (p<0.0001), Nox-2 (p<0.0001), Glycoprotein-VI (p<0.0001) and sP-selectin (p<0.0001) serum levels. Higher serum endocan levels were found with the worsening of metabolic profile (p<0.0001), specifically, NGT≥155 patients presented higher serum endocan values compared to NGT<155 (p<0.0001). From multivariate linear regression analysis, 1-h glucose resulted the major predictor of e-GFR justifying 23.6% of its variation (p<0.0001), 8-isoprostane and Nox-2 added respectively another 6.0% (p<0.0001) and 3.2% (p=0.001).Our study confirmed the link between 1-hour post-load glucose ≥155 mg/dl during OGTT and the possible increased risk for CKD, in newly diagnosed patients. The novelty is that we demonstrated a progressive increase in oxidative stress, platelets activation and serum endocan levels with the worsening of metabolic profile, which becomes evident early during the progression of CKD.