AUTHOR=Yin Shuyuan , Li Fenghua , Pan Zhennan , Bao Hongchu , Liu Xiaoyan 

TITLE=Alpha-lipoic acid reduces oxidative damage and ameliorates follicular abnormalities in vitrified cat ovarian tissue

JOURNAL=Frontiers in Endocrinology

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2025.1682526

DOI=10.3389/fendo.2025.1682526

ISSN=1664-2392

ABSTRACT=IntroductionVitrification may induce oxidative damage, mitigating cryodamage is crucial for preserving follicular development and ovarian function. Alpha lipoic acid (α-ALA) is an antioxidant which exerts its antioxidant activity directly or indirectly.MethodsIn this study, we established a α-ALA concentration gradients (0, 100, and 150 μM) in the vitrification solution, assessed follicular abnormalities through hematoxylin and eosin (HE), analyzed cell apoptosis via TUNEL staining analysis, immunohistochemistry (IHC) and Masson staining, explored differential genes by transcriptome sequencing.ResultsOur findings revealed that α-ALA alleviated oxidative stress damage and decreased follicular abnormalities induced by vitrification, enhanced antioxidant capacity and delayed the onset of fibrosis during in vitro culture. Moreover, our analysis revealed the effects of α-ALA on vitrification at the transcriptomic level.DiscussionIn this study, we focused on the anti-apoptotic effects of α-ALA in cat ovarian cortex vitrification, and concluded that α-ALA effectively mitigates vitrification correlated ovarian functional impairment.