AUTHOR=Yun Jang-Hyuk , Kim Hyun-Ouk , Jeong Jae-Hun , Min Yerin , Park Kwang-Hyun , Si Chuanling , Choi Sun-Eun 

TITLE=Ulmus davidiana var. japonica Extracts Suppress Lipopolysaccharide-Induced Apoptosis Through Intracellular Calcium Modulation in U937 Macrophages

JOURNAL=Frontiers in Energy Research

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/energy-research/articles/10.3389/fenrg.2022.820330

DOI=10.3389/fenrg.2022.820330

ISSN=2296-598X

ABSTRACT=This study was conducted to examine the anti-apoptotic effects of Ulmus davidiana extracts by regulation the intracellular cation mobilization in U937 human monocytic cells. To investigate modulatory effects on LPS-induced apoptosis and Ca2+ signaling pathway, we measured intracellular Ca2+ levels and various protein markers such as Bax, Bcl-2 and PARP. To isolate biopotent molecules, branches from U. davidiana were processed sequentially by 60% ethanol, supercritical fluid extraction, and ethyl acetate extraction of the remaining sample to obtain single fractions as well as ultimately catechin-glycoside which is one of the known bioeffector molecules of U. davidiana. LPS increased intracellular Ca2+ mobilization in U937 cells with transient oscillation and markedly increased Bax and PARP protein expression; however Bcl-2 was decreased. Each of the U. davidiana var. extracts as well as catechin-glycoside, significantly reduced LPS-induced intracellular Ca2+ mobilization and apoptosis-related molecules were also downregulated. These results suggest that U.  davidiana and catechin-glycoside may be useful for improving immune system function.