AUTHOR=Haq Abdul , Laiq Ur Rehman Mian , Rana Qurrat ul Ain , Khan Alam , Sajjad Wasim , Khan Haji , Khan Samiullah , Shah Aamer Ali , Hasan Fariha , Ahmed Safia , Islam Arshad , Badshah Malik , Shah Tawaf Ali , Dawoud Turki M. , Bourhia Mohammed TITLE=Production, optimization, and physicochemical characterization of biodiesel from seed oil of indigenously grown Jatropha curcas JOURNAL=Frontiers in Energy Research VOLUME=Volume 11 - 2023 YEAR=2023 URL=https://www.frontiersin.org/journals/energy-research/articles/10.3389/fenrg.2023.1225988 DOI=10.3389/fenrg.2023.1225988 ISSN=2296-598X ABSTRACT=98% biodiesel at optimized conditions: oil to methanol molar ratio 1:6, KOH 1%, time 90 minutes, and temperature 60 °C. Similarly, Bacillus subtilis Q5 strain yielded ~ 98% biodiesel at optimized condition: oil: methanol ratio of 1:9, agitation 150 RPM, inoculum size 10%, temperature 37 °C and n-hexane 10%. Moreover, factors such as oil-to-methanol molar ratio, amount of catalyst, time, and temperature were optimized and the highest volumetric biodiesel yield obtained was 98% at optimized conditions. The optimized conditions for maximum biodiesel yield were oil to methanol molar ratio 1:6, KOH 1%, time 90 minutes, and temperature 60 °C. The fuel properties of J. curcas seed biodiesel are closely related to standard values specified by American Society for Testing and Materials ( ASTM D6751-20a), indicating its potential as a viable biodiesel fuel source ASTM D6751.