AUTHOR=Jin Hui , Zhao Cheng , Yin Yi , Zheng Guangming , li Lichun , Shan Qi , Zhang Meiyu , Wei Linting , Shi Xiaona , Huang Heqing , Zhang Wenjing , Liu Shugui 

TITLE=Simultaneous Determination of Active Clinical Components of Teicoplanin and Ramoplanin in Environmental Water by LC-MS/MS Coupled With Cascade Elution

JOURNAL=Frontiers in Environmental Science

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/environmental-science/articles/10.3389/fenvs.2021.785408

DOI=10.3389/fenvs.2021.785408

ISSN=2296-665X

ABSTRACT=A simple, sensitive and simultaneous method was established and validated for active clinical components of teicoplanin and ramoplanin in environmental water by LC-MS/MS coupled with cascade elution. Moreover, a cascade elution method, which was rapid, solvent-less and high-extraction efficient, was successfully proposed to realize the extraction and purification of seven targets in one-step. Under the optimized conditions, the method showed excellent linearity with correlation coefficient (R2) ≥0.998 in the range of 1.0 ng L-1 to 100.0 ng L-1. Low matrix effects and good recoveries which ranged from 86 to 114% were reached with RSDs lower than 3.0% for most targets. The limits of detection and limit of quantification were 0.1-1.3 ng L-1 and 0.3-4.0 ng L-1, respectively. This method was successfully applied for the determination of teicoplanin and ramoplanin in water samples from the Pearl River and the South China Sea. TA2-2,3 was quantified in only one sample with the concentration of 8.0 ng L-1.