AUTHOR=Zolotukhina Anastasia , Machikhin Alexander , Guryleva Anastasia , Gresis Valeriya , Tedeeva Victoriya TITLE=Extraction of chlorophyll concentration maps from AOTF hyperspectral imagery JOURNAL=Frontiers in Environmental Science VOLUME=Volume 11 - 2023 YEAR=2023 URL=https://www.frontiersin.org/journals/environmental-science/articles/10.3389/fenvs.2023.1152450 DOI=10.3389/fenvs.2023.1152450 ISSN=2296-665X ABSTRACT=Remote measurement of chlorophyll concentration in leaves is highly important for multiple biological and agricultural applications. Due to precise, fast and arbitrary spectral tuning, acousto-optic (AO) imagers provide highly optimized data acquisition and processing. In this study, we demonstrate the feasibility to extract the distribution of chlorophyll content from AO hyperspectral data cubes. We collected spectral images of soybean leaves of 5 cultivars in the range 450-850 nm, calculated 14 different chlorophyll indices (CI), evaluated absolute value of chlorophyll concentration and compared it with well-established spectroscopic measurements. We found the most suitable CI and calculated chlorophyll maps from AO hyperspectral imaginary. This study exhibits high potential of AO imaging for real-time chlorophyll mapping.