AUTHOR=Pfaff Peter J. , Coulter Alison A. , Schall Benjamin J. , Davis Tanner , Chipps Steven R. , Coulter David P. TITLE=Predicting aquatic habitat connectivity across watershed boundaries: implications for interbasin spread of nonindigenous aquatic species JOURNAL=Frontiers in Environmental Science VOLUME=Volume 13 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/environmental-science/articles/10.3389/fenvs.2025.1646017 DOI=10.3389/fenvs.2025.1646017 ISSN=2296-665X ABSTRACT=Understanding habitat connectivity is critical for managing nonindigenous aquatic species (NAS) spread. Dams and watershed boundaries can be impassable to NAS during typical conditions but may become temporarily passable during flooding. The goal of our project was to develop an approach for identifying locations of aquatic connectivity at a fine spatial scale along watershed boundaries using readily available data. To develop this approach, we focused on the potential for range expansion of invasive fish in the United States via possible cross-boundary habitat connections. First, we developed an index using metrics of elevation, watershed size, and geology at regular points along a watershed boundary to stratify points by likelihood of connectivity during high precipitation (>20 mm of precipitation in a 3-day period). We then used a subset of points across a gradient of connectivity likelihoods to gather Landsat-derived observed surface water data and developed a statistical model to predict surface water presence from landscape characteristics. We applied the model throughout the entire watershed boundary to identify locations of hydrologic connectivity during high-water events. The presence of surface water on watershed boundaries was predicted by the interactions between watershed boundary point elevation relative to the minimum adjacent HUC-12 elevations and watershed boundary point elevation relative to neighboring point elevations (marginal R2 = 0.94). Our approach can be used to identify potential areas of surface water connectivity between watersheds quickly and easily at a fine spatial scale using readily available, remotely sensed data that can inform conservation and management actions across disciplines.