AUTHOR=Srivastava Malay , Kumari Moni , Karn Santosh Kumar , Bhambri Anne , Mahale Vivek G. , Mahale Sushma TITLE=Submerged cultivation and phytochemical analysis of medicinal mushrooms (Trametes sp.) JOURNAL=Frontiers in Fungal Biology VOLUME=Volume 5 - 2024 YEAR=2024 URL=https://www.frontiersin.org/journals/fungal-biology/articles/10.3389/ffunb.2024.1414349 DOI=10.3389/ffunb.2024.1414349 ISSN=2673-6128 ABSTRACT=Mushrooms are widely available around the world and have various nutritional as well as therapeutic values. The majority of Asian nations held the belief that medicinal mushrooms might prolong life and improve vitality. The study aims to characterize the phytochemical and polysaccharide, mainly β-glucan content of mycelial biomass and fruiting bodies collected from the Himalayan region, especially in Uttarakhand. Additionally, through molecular analysis of LSU F/ R-rDNA fragment sequence and phylogenetic analysis, this strain was identified as Trametes sp.We performed screening of phytochemicals and polysaccharides in mushroom and biomass extracts using the high-performance liquid chromatography (HPLC)method and PCbased (UV-Vis Spectrophotometer).The macrofungal biomass was high in saponin, anthraquinone, total phenolic, flavonoid, and β-glucan content.In biomass extract, we observed a high level of saponin (70.6µg/ml), followed by anthraquinone(14.5µg/ml), total phenolic(12.45 µg/ml), and flavonoid(9.500µg/ml) content.Furthermore, we examined the contents of alkaloids, tannins, terpenoids, and sterols in the biomass and mushroom extracts, the concentration of these compounds in the ethanol extract, tested was minimal. We also looked for Antioxidant activity which is determined in terms of IC 50 value, the Trametes sp. mushroom extract exhibits higher DPPH radical scavenging activity (62.9% at 0.5 mg/mL) than biomass extract (59.19 % at 0.5 mg/mL). We also analysed β-glucan in Trametes sp. from both mushroom and biomass extract,The biomass extract showed a higher β-glucan content of 1.713mg/ml than the mushroom extract which is 1.671 mg/ml. Further, β-glucan analysis was confirmed by Megazyme β-glucan assay kit from both biomass and mushroom extract ofTrametessp. β-glucans have a promising future in cancer treatment as an adjunct to conventional medicines. Producing pure β glucans for the market is challenging because 90-95% of β glucan sold nowadays is thought to be manipulated with or counterfeit. The present study results help recommend Trametes sp. rich in β-glucan, protein, phytochemicals, and antioxidant activities which help individuals associated with cancer, diabetes, obesity, etc.