AUTHOR=Meyer Katrina , Selbach Matthias 

TITLE=Quantitative affinity purification mass spectrometry: a versatile technology to study protein–protein interactions

JOURNAL=Frontiers in Genetics

VOLUME=Volume 6 - 2015

YEAR=2015

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2015.00237

DOI=10.3389/fgene.2015.00237

ISSN=1664-8021

ABSTRACT=While the genomic revolution has dramatically accelerated the discovery of disease-associated genes, the functional characterization of the corresponding proteins lags behind.  Most proteins fulfill their tasks in complexes with other proteins, and analysis of Protein-Protein Interactions (PPIs) can therefore provide insights into protein function. Several methods can be used to generate large-scale protein interaction networks. However, most of these approaches are not quantitative and therefore cannot reveal how perturbations affect the network. Here, we illustrate how a clever combination of quantitative mass spectrometry with different biochemical methods provides a rich toolkit to study different aspects of PPIs including topology, subunit stoichiometry, and dynamic behavior.