AUTHOR=Xia Xin , Ruan Yi , Li Boya , Yu Yansong , Kong Xiangbo , Zhuang Peilin , Wu Hong 

TITLE=The Long Non-coding RNA lnc-DMP1 Regulates Dmp1 Expression Through H3K27Ac Modification

JOURNAL=Frontiers in Genetics

VOLUME=Volume 11 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.00233

DOI=10.3389/fgene.2020.00233

ISSN=1664-8021

ABSTRACT=Several long noncoding RNAs (lncRNAs) have been reported regulate the expression of neighbor protein-coding genes at post-transcriptional, transcriptional and epigenetic levels. Dentin matrix protein 1 (DMP1), a noncollagenous extracellular matrix protein, plays an important role in dentin and bone mineralization. However, the transcriptional regulation of lncRNA on Dmp1 has not been reported. In this study, we identified a novel lncRNA named lnc-DMP1, which is near the Dmp1 gene region and undergoes remarkable changes during mandible development. Lnc-DMP1 is co-localized and significantly expressed correlation with Dmp1 in embryonic and postnatal mouse mandibles. In MC3T3-E1 cells, lnc-DMP1 positively regulates DMP1 expression and skeletal mineralization.Moreover, lnc-DMP1 induces the activity of Dmp1 promoter by modulating H3K27Ac enrichment in the promoter region of Dmp1. In conclusion, our results indicate that lnc-DMP1 is a novel lncRNA near the Dmp1 gene region and regulates the DMP1 expression by modulating the H3K27 acetylation level of the Dmp1 promoter.