AUTHOR=Nie Han , Zhang Kaihua , Xu Jiasheng , Liao Kaili , Zhou Weimin , Fu Zhonghua 

TITLE=Combining Bioinformatics Techniques to Study Diabetes Biomarkers and Related Molecular Mechanisms

JOURNAL=Frontiers in Genetics

VOLUME=Volume 11 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.00367

DOI=10.3389/fgene.2020.00367

ISSN=1664-8021

ABSTRACT=Objective: To explore the mechanism of plasma circulating miRNA-126 and miRNA-28-3p in diabetes mellitus (DM) patients，and to identify the related bioinformatics analysis． 
Methods: Randomly selected 120 DM patients as the observation group and 120 non- DM patients as the control group. The plasma circulating miRNA-126 and miRNA-28-3p were analyzed by qRT-PCR, and their target genes, biological information，related lncRNA and circRNA were predicted． 
Results: The circulating miRNA-126 (0.1162 ± 0.0236 vs 0.0018 ± 0.0862) and miRNA-28-3p (0.1378 ± 0.0268 vs 0.0006 ± 0.0167) levels in the observation group were significantly higher than those in the control group，and differences were statistically significant (P＜0.01). The Pearson correlation coefficient of miRNA-126 and miRNA- 28-3p was 0.4337 (P＜0.01). ROC curve analysis of miRNA-126 and  miRNA-28-3p showed that the differences of the area under curve were statistically significant between the two groups (P＜0.01). Bioinformatics prediction showed that miRNA-126 and miRNA-28-3p may be involved in regulation of the insulin signaling pathway，insulin receptor signaling pathway，insulin / insulin growth factor signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway and  angiogenesis. Moreover, it may be associated with a variety of lncRNA and cir-cRNA． 
Conclusion：Circulating miRNA-126 and miRNA-28-3p can be a potential biomarker of DM and it may play an important role in the DM by regulating insulin or insulin growth factor related signaling pathways．