AUTHOR=Belokopytova Polina , Fishman Veniamin TITLE=Predicting Genome Architecture: Challenges and Solutions JOURNAL=Frontiers in Genetics VOLUME=Volume 11 - 2020 YEAR=2021 URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.617202 DOI=10.3389/fgene.2020.617202 ISSN=1664-8021 ABSTRACT=Genome architecture plays a pivotal role in gene regulation. Use of the high throughput methods for chromatin profiling and 3D-interactions mapping provided rich experimental datasets describing genome organization and dynamics. These data challenges development of new models and algorithms connecting genome architecture with epigenetic marks and predicting changes of chromatin interactions during normal development and in course of diseases. In this review, we describe how chromatin architecture could be reconstructed from epigenetic data using biophysical or statistical approaches. We discuss the applicability and limitations of these methods for understanding mechanisms underlying chromatin architecture and scoring effects of structural variations in human cells.