AUTHOR=Ling Sunkai , He Yanru , Li Xiaoxue , Ma Yu , Li Yuan , Kong Bo , Huang Peilin 

TITLE=Significant Gene Biomarker Tyrosine Kinase Non-receptor 2 Mediated Cell Proliferation and Invasion in Colon Cancer

JOURNAL=Frontiers in Genetics

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.653657

DOI=10.3389/fgene.2021.653657

ISSN=1664-8021

ABSTRACT=Objective: The present research aimed to investigate the expression and biological function of TNK2 and miR-125a-3p in colon cancer.
Materials & methods: The expression of TNK2 and miR-125a-3p in colon cancer tissue samples was analyzed by Public databases including UALCAN and Oncomine. Then we verified their expression in colon cancer cell lines by qRT-PCR and Western Blot. By regulating the expression of TNK2 and miR-125a-3p in colon cancer cells, their functions and potential mechanisms were explored.
Results: The expression of TNK2 was overexpressed in colon cancer cell lines, and it was found to directly bind to miR-125a-3p, which was down-regulated in colon cancer cell lines. Their expressions can affect the proliferation and invasion of colon cancer. Additionally, Colon cancer patients with lower TNK2 expression had a better prognosis than those with higher TNK2 expression.
Conclusion: Our results demonstrated that TNK2 and miR-125a-3p played a critical role in colon cancer, and they could also be served as biomarkers and potential prognostic markers for colon cancer.