AUTHOR=Jin Shubo , Hu Yuning , Fu Hongtuo , Jiang Sufei , Xiong Yiwei , Qiao Hui , Zhang Wenyi , Gong Yongsheng , Wu Yan 

TITLE=Identification and Characterization of the Succinate Dehydrogenase Complex Iron Sulfur Subunit B Gene in the Oriental River Prawn, Macrobrachium nipponense

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.698318

DOI=10.3389/fgene.2021.698318

ISSN=1664-8021

ABSTRACT=<p>Previous studies have revealed that <italic>SDHB</italic> has potential functions in the male sexual differentiation and development in <italic>M</italic>. <italic>nipponense</italic> through providing ATP. In this study, the functions of <italic>Mn-SDHB</italic> were further analyzed in depth using quantitative polymerase chain reaction (qPCR), <italic>in situ</italic> hybridization, western-blot, and RNA interference (RNAi), combined with the histological observations. The full-genome sequence of <italic>Mn-SDHB</italic> was 54,608 bp at Chromosome 34, including 7 introns and 6 exons. The full-length cDNA sequence of <italic>Mn-SDHB</italic> was 1,268 base pairs (bp) long with an open reading frame of 807 bp, encoding for 268 amino acids. The highest expression level of <italic>Mn-SDHB</italic> in different tissues was observed in the testis, and male prawns at post-larval developmental stage 25 during different developmental stages, indicating that <italic>SDHB</italic> was potentially involved in the male sexual development in <italic>M</italic>. <italic>nipponense</italic>. <italic>In situ</italic> hybridization and western-blot analysis indicated that <italic>SDHB</italic> plays essential roles in the testis development. The <italic>in situ</italic> hybridization analysis also implies the potential roles of <italic>Mn-SDHB</italic> in ovarian development. The expressions of <italic>Mn-IAG</italic> were decreased after <italic>Mn-SDHB</italic> dsRNA injection, indicating <italic>SDHB</italic> has the positive regulatory effects on <italic>IAG</italic> in <italic>M</italic>. <italic>nipponese</italic>. Thus, <italic>SDHB</italic> was involved in the mechanism of the male sexual development. The testis development was inhibited, and sperms were rarely observed after 10 days of <italic>Mn-SDHB</italic> dsRNA injection, indicating <italic>SDHB</italic> has positive effects on the male sexual development in <italic>M</italic>. <italic>nipponense</italic>. This study highlights the functions of <italic>SDHB</italic> in <italic>M</italic>. <italic>nipponense</italic>, which provide new insights for the future studies of the male sexual development in other crustacean species.</p>