AUTHOR=Petry Bruna , Moreira Gabriel Costa Monteiro , Copola Aline Gonçalves Lio , Souza Marcela Maria de , da Veiga Fernanda Cristina , Jorge Erika Cristina , de Oliveira Peixoto Jane , Ledur Mônica Corrêa , Koltes James E. , Coutinho Luiz Lehmann TITLE=SAP30 Gene Is a Probable Regulator of Muscle Hypertrophy in Chickens JOURNAL=Frontiers in Genetics VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.709937 DOI=10.3389/fgene.2021.709937 ISSN=1664-8021 ABSTRACT=

Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy.