AUTHOR=Zhang Taofeng , Chen Yihuan , Lin Weixun , Zheng Jiehua , Liu Yiyuan , Zou Juan , Cai Jiehui , Chen Yaokun , Li Zhiyang , Chen Yexi 

TITLE=Prognostic and Immune-Infiltrate Significance of miR-222-3p and Its Target Genes in Thyroid Cancer

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.710412

DOI=10.3389/fgene.2021.710412

ISSN=1664-8021

ABSTRACT=<p>Thyroid cancer (THCA) is a common endocrine malignancy. With increasing incidence and low mortality, balancing the therapeutic approach is an inevitable issue. This study aimed to confirm the role of miR-222-3p and its target genes in THCA survival and immune infiltration. From different expression analyses based on the GEO and TCGA databases, we predicted and subsequently identified the key target genes of miR-222-3p. We then explored the expression, enrichment, pairwise correlation, protein expression, survival analysis, principal component analysis, and immune significance of the critical genes using bioinformatics analysis. The present study demonstrated that <italic>NEGR1</italic>, <italic>NTNG1</italic>, <italic>XPNPEP2</italic>, <italic>NTNG2</italic>, <italic>CD109</italic>, <italic>OPCML</italic>, and <italic>PRND</italic> are critical genes. The miR-222-3p was highly expressed, probably leading to low <italic>NEGR1</italic> and high <italic>PRND</italic> expression in THCA tissues. Low <italic>NEGR1</italic> expression indicated favorable prognosis in THCA patients, and high <italic>PRND</italic> expression indicated poor prognosis. Seven critical genes were significantly related to gender, age, race, tumor stage, and lymph node metastasis. In addition, the seven-gene biomarker exhibited a certain diagnostic value. Finally, <italic>CD109</italic> expression was closely correlated with immune cells, especially B cells and CD4<sup>+</sup> T cells. The miR-222-3p and its critical target genes could be promising biomarkers for the prognosis of THCA and may emerge as key regulators of immune infiltration in THCA.</p>