AUTHOR=Verma Preeti , Sharma Ankita , Sodhi Monika , Tiwari Manish , Vivek Prince , Kataria Ranjit S. , Nirajan S. K. , Bharti Vijay K. , Singh Pawan , Lathwal S. S. , Sharma Vishal , Masharing Nampher , Mukesh Manishi 

TITLE=Identification of Internal Reference Genes in Peripheral Blood Mononuclear Cells of Cattle Populations Adapted to Hot Arid Normoxia and Cold Arid Hypoxia Environments

JOURNAL=Frontiers in Genetics

VOLUME=Volume 12 - 2021

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.730599

DOI=10.3389/fgene.2021.730599

ISSN=1664-8021

ABSTRACT=For accurate and reliable gene expression, the quantitative real-time polymerase chain reaction data requires normalization with a reference gene expressed constantly under all the experimental conditions being analyzed. Till date, no comparative data on suitable RGs are available for cattle populations reared at cold arid high-altitude hypoxia and hot arid tropical normoxia environment. Therefore, the present study was aimed to assess the expression stability of 10 RGs (GAPDH, RPL4, EEF1A1, RPS9, HPRT1, UXT, HMBS, B2M, RPS15, ACTB) in PBMCs of cattle types from cold arid high-altitude hypoxia and hot arid normoxia environments. Four different statistical algorithms: geNorm, NormFinder, BestKeeper and RefFinder were used to assess the stability of these genes.  A total of 30 blood samples were collected; 6 adult heifers each of Ladakhi (LAC), Holstein Frisian crosses (HFX) and 4 of Jersey (JYC) cows from cold arid high-altitude hypoxia environment (Group I) and 5 adult heifers each of Sahiwal (SAC), Karan Fries (KFC), Holstein Friesian (HFC) cows from hot arid normoxia environment (Group II). Combined analysis of group I and group II resulted in identification of panel of RGs like RPS9, RPS15, GAPDH that could act as a useful resource to unravel the accurate transcriptional profile of peripheral blood mononuclear cells of cattle populations adapted to tropical and high-altitude conditions.