AUTHOR=Wang Jie , Wang Meigui , Shao Jiahao , Liu Zheliang , Fu Chong , Chen Guanhe , Zhao Kaisen , Li Hong , Sun Wenqiang , Jia Xianbo , Chen Shiyi , Lai Songjia 

TITLE=Combined analysis of differentially expressed lncRNAs and miRNAs in liver tissues of high-fat fed rabbits by transcriptome sequencing

JOURNAL=Frontiers in Genetics

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.1000574

DOI=10.3389/fgene.2022.1000574

ISSN=1664-8021

ABSTRACT=High-fat diet could lead to a series of metabolic diseases, including obesity, and its mechanism is not clear. In this study, the rabbit individuals were fed with high-fat diet, the liver tissues were collected, high-throughput sequencing technology was used to reveal the expression of lncRNA and miRNA difference, and the molecular regulation mechanism of lncRNA-miRNA. A total of 24615 DE lncRNAs and 52 DE miRNAs were identified, including 15 novel discovered DE miRNAs (5 up-regulated and 10 down-regulated). GO and KEGG analyzed 757139 target genes respectively, enriching the pathways related to lipid metabolism, including mucin O-glycan biosynthesis pathway, insulin resistance and glucagon signaling pathway. Moreover, 78 targeting relationships were obtained. Among them, miR-375-5p/LOC108175519, miR-181d-3p/LOC108178671, miR-450a-5p/LOC108176670, miR-135a-3p/LOC10817690, miR-450a-3p/LOC103345690 were proposed the first time. Significantly, miR-450a-5p/LOC108176670 and miR-135a-3p/LOC10817690 were related to lipid metabolism in the liver. This study provides a new idea for the study of steatosis caused by high-fat and a new perspective for gene therapy.