AUTHOR=Wu Wenqi , Liu Su , Tian Linyan , Li Cheng , Jiang Yanan , Wang Jinhuan , Lv Yangyang , Guo Jing , Xing Donghui , Zhai Yixin , Sun Huimeng , Li Yuhang , Zhang Luying , He Xiang , Luo Kaiping , Zhan Hongjie , Zhao Zhigang 

TITLE=Identification of microtubule-associated biomarkers in diffuse large B-cell lymphoma and prognosis prediction

JOURNAL=Frontiers in Genetics

VOLUME=Volume 13 - 2022

YEAR=2023

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.1092678

DOI=10.3389/fgene.2022.1092678

ISSN=1664-8021

ABSTRACT=<p><bold>Background:</bold> Diffuse large B-cell lymphoma (DLBCL) is a genetically heterogeneous disease with a complicated prognosis. Even though various prognostic evaluations have been applied currently, they usually only use the clinical factors that overlook the molecular underlying DLBCL progression. Therefore, more accurate prognostic assessment needs further exploration. In the present study, we constructed a novel prognostic model based on microtubule associated genes (MAGs).</p><p><bold>Methods:</bold> A total of 33 normal controls and 1360 DLBCL samples containing gene-expression from the Gene Expression Omnibus (GEO) database were included. Subsequently, the univariate Cox, the least absolute shrinkage and selection operator (LASSO), and multivariate Cox regression analysis were used to select the best prognosis related genes into the MAGs model. To validate the model, Kaplan-Meier curve, and nomogram were analyzed.</p><p><bold>Results:</bold> A risk score model based on fourteen candidate MAGs (<italic>CCDC78, CD300LG, CTAG2, DYNLL2, MAPKAPK2, MREG, NME8, PGK2, RALBP1, SIGLEC1, SLC1A1, SLC39A12, TMEM63A, and WRAP73</italic>) was established. The K-M curve presented that the high-risk patients had a significantly inferior overall survival (OS) time compared to low-risk patients in training and validation datasets. Furthermore, knocking-out <italic>TMEM63A</italic>, a key gene belonging to the MAGs model, inhibited cell proliferation noticeably.</p><p><bold>Conclusion:</bold> The novel MAGs prognostic model has a well predictive capability, which may as a supplement for the current assessments. Furthermore, candidate TMEM63A gene has therapeutic target potentially in DLBCL.</p>