AUTHOR=Nedoluzhko Artem , Sharko Fedor , Tsygankova Svetlana , Boulygina Eugenia , Slobodova Natalia , Teslyuk Anton , Galindo-Villegas Jorge , Rastorguev Sergey 

TITLE=Intergeneric hybridization of two stickleback species leads to introgression of membrane-associated genes and invasive TE expansion

JOURNAL=Frontiers in Genetics

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.863547

DOI=10.3389/fgene.2022.863547

ISSN=1664-8021

ABSTRACT=<p>Interspecific hybridization has occurred relatively frequently during the evolution of vertebrates. This process usually abolishes reproductive isolation between the parental species. Moreover, it results in the exchange of genetic material and can lead to hybridogenic speciation. Hybridization between species has predominately been observed at the interspecific level, whereas intergeneric hybridization is rarer. Here, using whole-genome sequencing analysis, we describe clear and reliable signals of intergeneric introgression between the three-spined stickleback (<italic>Gasterosteus aculeatus</italic>) and its distant mostly freshwater relative the nine-spined stickleback (<italic>Pungitius pungitius</italic>) that inhabit northwestern Russia. Through comparative analysis, we demonstrate that such introgression phenomena apparently take place in the moderate-salinity White Sea basin, although it is not detected in Japanese sea stickleback populations. Bioinformatical analysis of the sites influenced by introgression showed that they are located near transposable elements, whereas those in protein-coding sequences are mostly found in membrane-associated and alternative splicing-related genes.</p>