AUTHOR=Wang Yu , Wang Zhen , Li KaiQiang , Xiang WeiLing , Chen BinYu , Jin LiQin , Hao Ke 

TITLE=lncRNAs Functioned as ceRNA to Sponge miR-15a-5p Affects the Prognosis of Pancreatic Adenocarcinoma and Correlates With Tumor Immune Infiltration

JOURNAL=Frontiers in Genetics

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.874667

DOI=10.3389/fgene.2022.874667

ISSN=1664-8021

ABSTRACT=Pancreatic adenocarcinoma(PAAD) is one of the most common malignant tumors with a poor prognosis worldwide. Mounting evidence suggests that the expression of lncRNAs and the infiltration of immune cells have prognostic value for patients with PAAD. We used GENE EXPRESSION OMNIBUS(GEO)database and identified six genes (COL1A2, ITGA2, ITGB6, LAMA3, LAMB3, LAMC2) that could affect the survival rate of Pancreatic adenocarcinoma patients. Based on a series of in silico analyses for reverse prediction of target genes associated with the prognosis of PAAD, a ceRNA network of mRNA (COL1A2, ITGA2, LAMA3, LAMB3, LAMC2)-microRNA (miR-15a-5p)-long non-coding RNA (LINC00511, LINC01578, PVT1, TNFRSF14-AS1) was constructed. We used the algorithm “CIBERSORT” to assess the proportion of immune cells and found three OS-associated immune cells (Monocytes, Macrophages M1, and Mast cell resting). Moreover, OS-associated genes level was significantly positively associated with immune checkpoint expression and biomarkers of immune cells. In summary, our results clarified that ncRNAs-mediated upregulation of OS-associated genes, and tumor-infiltration immune cells (Monocytes, Macrophages M1, and Mast cell resting) correlated with poor prognosis in PAAD.