AUTHOR=Wang Cui , Zhang Xunjie , Zeng Zhiheng , Song Feifei , Lin Zhen , Chen Liangjun , Cai Zhixin 

TITLE=Transcriptome Analysis Explored the Differential Genes’ Expression During the Development of the Stropharia rugosoannulata Fruiting Body

JOURNAL=Frontiers in Genetics

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.924050

DOI=10.3389/fgene.2022.924050

ISSN=1664-8021

ABSTRACT=Stropharia rugosoannulata (S. rugosoannulata) is a fungus with great edible and nutritional value, while the development mechanism of its fruiting body has not been revealed. Thus, this study aimed to analyze the differentially expressed genes (DEGs) in the four stages; primordia stage (Sra1), young mushroom stage (Sra2), picking stage (Sra3) and opening umbrella stage (Sra4). Therefore, total RNA have been extracted for further RNA sequencing analysis. In three pairwise comparison groups (PCGs), Sar1 vs Sar2, Sar2 vs Sar3, Sar3 vs Sar4, a total of 3112 DEGs were identified among three PCGs. GO analysis of DEGs showed that there were 21 terms significantly enriched in Sra1vs Sra2 PCG. There was no significantly enriched GO term in the other two PCGs. Further KEGG pathway analysis showed that these DEGs were mainly enriched in glucose and amino acid metabolism. Moreover we found intron retention (IR) and alternative 3' splice site (A3SS) accounted for more than 80%. The development of S. rugosoannulata fruiting body mainly involved in glucose and amino acid metabolism. IR and A3SS were the two main types of ASE, which played an important role in the development and maturation of S. rugosoannulata fruiting body.