AUTHOR=Wang Fengfeng , Cheung Chi Wai , Wong Stanley Sau Ching 

TITLE=Use of pain-related gene features to predict depression by support vector machine model in patients with fibromyalgia

JOURNAL=Frontiers in Genetics

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2023.1026672

DOI=10.3389/fgene.2023.1026672

ISSN=1664-8021

ABSTRACT=The prevalence rate of depression is higher in patients with fibromyalgia syndrome (FMS), but this is often unrecognized in patients with chronic pain. Given that depression is a common major barrier in the management of patients with FMS, an objective tool that reliably predicts depression in patients with FMS could significantly enhance the diagnostic accuracy. Since pain and depression can cause each other and worsen each other, we wonder if pain-related genes can be used to predict depression. This study developed a support vector machine (SVM) model combined with principal component analysis (PCA) to predict depression in FMS patients using a microarray dataset, including 25 FMS patients with major depression, and 36 patients without major depression. Gene co-expression analysis was used to select gene features to construct SVM model. PCA can help reduce the number of data dimensions without much loss of information, and identify patterns in data easily. The 61 samples available in the database were not enough for learning based methods and cannot represent every possible variation of each patient. To address this issue, we adopted Gaussian noise to generate a large amount of simulated data for training and testing of the model. The ability of SVM model to predict depression using microarray data was measured as accuracy. Different structural co-expression patterns were identified for 114 genes involved in pain signaling pathway by two-sample KS test (p<0.001 for the maximum deviation D = 0.11 > Dcritical = 0.05), indicating the aberrant co-expression patterns in FMS patients. Twenty hub gene features were further selected based on co-expression analysis to construct the model. PCA reduced the dimension of the training samples from 20 to 16, since 16 components were needed to retain more than 90% of the original variance. The SVM model was able to identify depression in FMS patients with an average accuracy of 93.22% (3.59) based on the expression levels of the selected hub gene features. These findings would contribute key information that can be used to develop a clinical decision-making tool for the data-driven, personalized optimization of diagnosing depression in patients with FMS.