AUTHOR=Liu Mengfeng , Yu Xiran , Bu Jianlong , Xiao Qifan , Ma Sitong , Chen Naozhong , Qu Changfa 

TITLE=Comparative analyses of salivary exosomal miRNAs for patients with or without lung cancer

JOURNAL=Frontiers in Genetics

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2023.1249678

DOI=10.3389/fgene.2023.1249678

ISSN=1664-8021

ABSTRACT=Lung cancer is the most frequent cause of cancer-related deaths worldwide.Exosomes are involved in different types of cancer, including lung cancer. We collected saliva from persons with (LC) or without (NC) lung cancer, and successfully isolated salivary exosomes by ultracentrifugation. The miRNA sequencing was implemented for the exosome samples from NC and LC groups, and a total of 372 miRNAs were found. Subsequently, 15 differentially expressed(DE) miRNAs were identified in LC vs NC, including 8 upregulated and 7 downregulated miRNAs. Some DE miRNAs were validated by quantitative real time polymerase chain reaction (qPCR). A total of 488 putative target genes of the upregulated DE miRNAs were found, and the functional analyses indicated that numerous target genes were enriched in the pathways associated with cancer, suggesting that miRNAs of salivary exosomes might have the potential to be used as biomarkers for prediction and diagnosis of lung cancer.