AUTHOR=Shen Chunxiu , Lu Qineng , Yang Di , Zhang Xueru , Huang Xinping , Li Rungen , Que Zhiqun , Chen Na 

TITLE=Genome-wide identification analysis in wild-type Solanum pinnatisectum reveals some genes defending against Phytophthora infestans

JOURNAL=Frontiers in Genetics

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2024.1379784

DOI=10.3389/fgene.2024.1379784

ISSN=1664-8021

ABSTRACT=Solanum Pinnatisectum exhibits strong resistance to late blight due to Phytophthora infestans, but only an incomplete genome assembly based on short Illumina reads has been published.In this study, we generated the first chromosome-level draft genome for the wild-type potato species S. Pinnatisectum in China using Oxford Nanopore Technology (ONT) sequencing and Hi-C technology. The high-quality assembled genome size is 664 Mb with a scaffold N50 value of 49.17 Mb, 65.87% of which was occupied by repetitive sequences, and long terminal repeats (LTRs) were predominant (42.51% of the whole genome). The genome of S.Pinnatisectum was predicted to contain 34,245 genes, and 99.34% of which were functionally annotated. Moreover, 303 NBS-coding disease resistance (R) genes were predicted in the S.Pinnatisectum genome to investigate potential mechniasms of late blight disease resistance.The high quality chromosome-level reference S. Pinnatisectum genome may provide potential valuable resources for intensively and effectively investigating molecular breeding and genetic research in the future.