AUTHOR=Deng Wangdong , Cai Xinyi 

TITLE=Complete mitochondrial genome of Ficus hirta and its comparative analysis

JOURNAL=Frontiers in Genetics

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1530105

DOI=10.3389/fgene.2025.1530105

ISSN=1664-8021

ABSTRACT=IntroductionFicus hirta, known as Wuzhimaotao in China, is a dioecious plant species within the Moraceae family, highly regarded for its medicinal and ecological roles.MethodsUtilizing a hybrid assembly methodology, combining Nanopore and Illumina sequencing, we achieved a detailed mitochondrial genomic architecture.ResultsThe genome exhibits single circular structures, spans 486,226 base pairs with 45.21% GC content, and encompasses 31 distinct protein-coding genes.Our analysis extends to gene content, codon usage, intergenomic transfers, phylogenetic relationships, and RNA editing patterns. Notably, the mitochondrial and chloroplast genomes share 15 homologous fragments, underpinning intercellular gene exchange. Phylogenetic positioning confirms Ficus hirta within Moraceae, closely allied with Morus notabilis.DiscussionThis comprehensive mitochondrial genome elucidation not only augments the biological understanding of Ficus hirta but also enriches genomic resources for future research.