AUTHOR=Wang Yi-Ran , Du Pu-Feng 

TITLE=WCSGNet: a graph neural network approach using weighted cell-specific networks for cell-type annotation in scRNA-seq

JOURNAL=Frontiers in Genetics

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1553352

DOI=10.3389/fgene.2025.1553352

ISSN=1664-8021

ABSTRACT=Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for understanding cellular heterogeneity, providing unprecedented resolution in molecular regulation analysis. Existing supervised learning approaches for cell type annotation primarily utilize gene expression profiles from scRNA-seq data. Although some methods incorporated gene interaction network information, they fail to use cell-specific gene association networks. This limitation overlooks the unique gene interaction patterns within individual cells, potentially compromising the accuracy of cell type classification. We introduce WCSGNet, a graph neural network-based algorithm for automatic cell-type annotation that leverages Weighted Cell-Specific Networks (WCSNs). These networks are constructed based on highly variable genes and inherently capture both gene expression patterns and gene association network structure features. Extensive experimental validation demonstrates that WCSGNet consistently achieves superior cell type classification performance, ranking among the top-performing methods while maintaining robust stability across diverse datasets. Notably, WCSGNet exhibits a distinct advantage in handling imbalanced datasets, outperforming existing methods in these challenging scenarios. All datasets and codes for reproducing this work were deposited in a GitHub repository (https://github.com/Yi-ellen/WCSGNet).