AUTHOR=Huang Xindi , Jiang Jipu , Shi Lifen , Yan Cheng 

TITLE=GTMALoc: prediction of miRNA subcellular localization based on graph transformer and multi-head attention mechanism

JOURNAL=Frontiers in Genetics

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1623008

DOI=10.3389/fgene.2025.1623008

ISSN=1664-8021

ABSTRACT=MicroRNAs (miRNAs) play a crucial role in regulating gene expression, and their subcellular localization is essential for understanding their biological functions. However, accurately predicting miRNA subcellular localization remains a challenging task due to their short sequences, complex structures, and diverse functions. To improve prediction accuracy, this study proposes a novel model based on a graph transformer and a multi-head attention mechanism. The model integrates multi-source features which include the miRNA sequence similarity network, miRNA functional similarity network, miRNA–mRNA association network, miRNA–drug association network, and miRNA–disease association network. Specifically, we first apply the node2vec algorithm to extract features from these biological networks. Then, we use a graph transformer to capture relationships between nodes within the networks, enabling a better understanding of miRNA functions across different biological contexts. Next, a multi-head attention mechanism is implemented to combine miRNA features from multiple networks, allowing the model to capture deeper feature relationships and enhance prediction performance. Performance evaluation shows that the proposed method achieves significant improvements over current approaches on open-access datasets, achieving high performance with an AUC (area of receiver operating characteristic curve) of 0.9108 and AUPR(area of precision-recall curve) of 0.8102. It not only significantly improves prediction accuracy but also exhibits strong generalization and stability.