AUTHOR=Koo Kyo-Chan 

TITLE=Rapid forensic ancestry inference in selected Northeast Asian populations: a Y-STR based attention-based ensemble framework for initial investigation guidance

JOURNAL=Frontiers in Genetics

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1631529

DOI=10.3389/fgene.2025.1631529

ISSN=1664-8021

ABSTRACT=IntroductionRapid inference of ancestral origin fromDNA evidence is critical in time-sensitive forensic investigations, particularly during the initial hours when crucial investigative decisions must be made. Although comprehensive analyses using multiple genetic markers provide thorough results, they often require significant processing time and resources. Y-chromosome short tandem repeats (Y-STRs) exhibit population-specific allelic distributions that facilitate rapid analysis, making them particularly valuable for initial screening in forensic contexts.MethodsThis study aims to enhance population classification accuracy using Y-STR profile analysis, with a particular focus on Northeast Asian populations that are often merged into a single group by commercial ancestry panels. We developed a machine learning architecture centered on an attention-based ensemble mechanism that incorporates three complementary algorithms: a One-vs-Rest Random Forest, XGBoost, and Logistic Regression, each configured to effectively manage imbalanced datasets.ResultsUtilizing only Y-STR data, the model achieved an overall accuracy of 80%-81% and demonstrated high stability. Notably, the model effectively processes imbalanced datasets, generating reliable outcomes for rapid ancestry assessment in time-critical investigations.DiscussionBy addressing a key limitation in commercial ancestry panels--their failure to differentiate among Northeast Asian subpopulations--this framework provides valuable preliminary guidance in forensic cases involving Asian individuals. Consequently, our approach enhances rapid screening capabilities, which can inform early-stage investigations while complementing subsequent, more comprehensive genetic analyses.