AUTHOR=Tannous Joanna , Sawyer Cole , Hassan Md Mahmudul , Labbe Jesse L. , Eckert Carrie TITLE=Establishment of a genome editing tool using CRISPR-Cas9 ribonucleoprotein complexes in the non-model plant pathogen Sphaerulina musiva JOURNAL=Frontiers in Genome Editing VOLUME=5 YEAR=2023 URL=https://www.frontiersin.org/journals/genome-editing/articles/10.3389/fgeed.2023.1110279 DOI=10.3389/fgeed.2023.1110279 ISSN=2673-3439 ABSTRACT=
CRISPR-Cas9 is a versatile genome editing system widely used since 2013 to introduce site-specific modifications into the genomes of model and non-model species. This technology is used in various applications, from gene knock-outs, knock-ins, and over-expressions to more precise changes, such as the introduction of nucleotides at a targeted locus. CRISPR-Cas9 has been demonstrated to be easy to establish in new species and highly efficient and specific compared to previous gene editing strategies such as Zinc finger nucleases and transcription activator-like effector nucleases. Grand challenges for emerging CRISPR-Cas9 tools in filamentous fungi are developing efficient transformation methods for non-model organisms. In this paper, we have leveraged the establishment of CRISPR-Cas9 genome editing tool that relies on Cas9/sgRNA ribonucleoprotein complexes (RNPs) in the model species