AUTHOR=Yao Zhi Q. , Schank Madison B. , Zhao Juan , El Gazzar Mohamed , Wang Ling , Zhang Yi , Hill Addison C. , Banik Puja , Pyburn Jaeden S. , Moorman Jonathan P. 

TITLE=The potential of HBV cure: an overview of CRISPR-mediated HBV gene disruption

JOURNAL=Frontiers in Genome Editing

VOLUME=Volume 6 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/genome-editing/articles/10.3389/fgeed.2024.1467449

DOI=10.3389/fgeed.2024.1467449

ISSN=2673-3439

ABSTRACT=This article reviewed the CRISPR/Cas9 technology to disrupt and inactivate HBV genome.We summarized the tools employed in designing guide RNAs (gRNAs) targeting HBV genomes, the vehicles used for expressing and delivering CRISPR/Cas9 components, the models used for evaluating CRISPR-mediated HBV gene disruption, the methods used for assessing antiviral and off-target effects induced by CRISPR/Cas9, and the prospects of future directions and challenges in leveraging this HBV gene-editing approach to advance the HBV treatment toward a clinical cure.