AUTHOR=Wang Yajie , Zhu Chunyue , Zhou Junhong , Fu Tianli , Yan Jinlong , Wang Bangyu , Lü Jiaojiao , Huang Lingyan , Liu Yu 

TITLE=20 Hz temporal interference stimulation can more effectively enhance motor evoked potentials in the primary motor cortex

JOURNAL=Frontiers in Human Neuroscience

VOLUME=Volume 19 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/human-neuroscience/articles/10.3389/fnhum.2025.1524485

DOI=10.3389/fnhum.2025.1524485

ISSN=1662-5161

ABSTRACT=ObjectiveThis study investigates the effects of temporal interference stimulation (TI) with different envelope frequencies on the cortical excitability of the primary motor cortex (M1).MethodsIn this randomized, double-blind, crossover study, 26 participants completed four separate study visits. During these visits, they received 20 min of three types of TI (10, 20, and 40 Hz envelope frequency) and sham stimulation applied over M1 in a randomized order. Transcranial magnetic stimulation (TMS) was employed to assess motor-evoked potentials (MEP) and resting motor threshold (RMT) over the left M1 (ipsilateral area) and right M1 (contralateral area) before, immediately after, and 30 and 60 min after stimulation.ResultsThe blinding efficacy was excellent, and no severe side effects were reported. TI stimulation with varying envelope frequencies affected MEP differently; 20 Hz TI stimulation enhanced the MEP of the ipsilateral M1 with after-effects appearing at 60 min, and no significant differences were observed between the 10 or 20 Hz TI stimulation with sham groups. However, no significant changes in RMT were observed under any of the TI conditions.Conclusion20 Hz TI stimulation increased the cortical excitability of the ipsilateral M1, highlighting that frequency is an important factor in the modulatory effect of TI.