AUTHOR=Alvarez Salazar Evelyn Katy , Cortés-Hernández Arimelek , Alemán-Muench Germán Rodrigo , Alberú Josefina , Rodríguez-Aguilera Jesús R. , Recillas-Targa Félix , Chagoya de Sánchez Victoria , Cuevas Eric , Mancilla-Urrea Eduardo , Pérez García María , Mondragón-Ramírez Guillermo , Vilatobá Mario , Bostock Ian , Hernández-Méndez Erick , De Rungs David , García-Zepeda Eduardo A. , Soldevila Gloria 

TITLE=Methylation of FOXP3 TSDR Underlies the Impaired Suppressive Function of Tregs from Long-term Belatacept-Treated Kidney Transplant Patients

JOURNAL=Frontiers in Immunology

VOLUME=Volume 8 - 2017

YEAR=2017

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2017.00219

DOI=10.3389/fimmu.2017.00219

ISSN=1664-3224

ABSTRACT=Regulatory T cells are considered key players in the prevention of allograft rejection in transplanted patients. Belatacept, is an effective alternative to calcineurin inhibitors that appears to preserve graft survival and function, however, the impact of this drug in the homeostasis of Tregs in transplanted patients remains controversial. Here we analyzed the phenotype, function and the epigenetic status of the Treg-specific demethylated region (TSDR) in FOXP3 of circulating Tregs from long-term kidney transplant patients under Belatacept or Cyclosporine A treatment. We found a significant reduction in the proportion of CD4+CD25hiCD127lo/-FOXP3+ T cells in all patients compared to healthy individual (controls). Interestingly, only Belatacept-treated patients displayed an enrichment of the CD45RA+ “naïve” Tregs, while the expression of Helios, a marker used to identify stable FOXP3+ thymic Tregs remained unaffected. Functional analysis demonstrated that Tregs from transplanted patients displayed a significant reduction in their suppressive capacity compared to Tregs from controls, which is associated with decreased levels of FOXP3 and CD25. Analysis of the methylation status of the FOXP3 gene showed that Belatacept treatment results in methylation of CpG islands within the TSDR, which could be associated with the impaired Treg suppression function. Our data indicate that analysis of circulating Tregs cannot be used as a marker for assessing tolerance towards the allograft in long-term kidney transplant patients.