AUTHOR=Riou Catherine , Berkowitz Natacha , Goliath Rene , Burgers Wendy A. , Wilkinson Robert J. 

TITLE=Analysis of the Phenotype of Mycobacterium tuberculosis-Specific CD4+ T Cells to Discriminate Latent from Active Tuberculosis in HIV-Uninfected and HIV-Infected Individuals

JOURNAL=Frontiers in Immunology

VOLUME=Volume 8 - 2017

YEAR=2017

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2017.00968

DOI=10.3389/fimmu.2017.00968

ISSN=1664-3224

ABSTRACT=Several immune-based assays have been suggested to differentiate latent from active tuberculosis. However, their relative performance as well as their efficacy in HIV-infected persons, a highly at-risk population, remains unclear. 
	In a study of 81 individuals, divided into four groups based on their HIV-1 status and TB disease activity, we compared the differentiation (CD27, KLRG1), activation (HLA-DR), homing potential (CCR4, CCR6, CXCR3, CD161) and functional profiles (IFNγ, IL-2 and TNFα) of Mycobacterium tuberculosis (Mtb)-specific CD4+ T cells using flow cytometry.
	Active TB disease induced major changes within the Mtb-responding CD4+ T cell population, promoting memory maturation, elevated activation and increased inflammatory potential when compared to individuals with latent TB infection. Moreover, the functional profile of Mtb-specific CD4+ T cells appeared to be inherently related to their degree of differentiation. While these specific cell features were all capable of discriminating latent from active TB, irrespective of HIV status, HLA-DR expression showed the best performance for TB diagnosis (AUC=0.92, 95% CI: 0.82-1.01, specificity: 82%, sensitivity: 84% for HIV- and AUC=0.99, 95% CI: 0.98-1.01, specificity: 94%, sensitivity: 93% for HIV+).
	In conclusion, these data support the idea that analysis of T cell phenotype can be diagnostically useful in TB.