AUTHOR=Chen Lin , Wang Chengmin , Luo Jing , Su Wen , Li Meng , Zhao Na , Lyu Wenting , Attaran Hamidreza , He Yapeng , Ding Hua , He Hongxuan 

TITLE=Histone Deacetylase 1 Plays an Acetylation-Independent Role in Influenza A Virus Replication

JOURNAL=Frontiers in Immunology

VOLUME=Volume 8 - 2017

YEAR=2017

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2017.01757

DOI=10.3389/fimmu.2017.01757

ISSN=1664-3224

ABSTRACT=Influenza A viruses (IAVs) take advantage of the host acetylation system for their own benefit. Whether the nucleoprotein (NP) of influenza A viruses undergoes acetylation and the interaction between the nucleoprotein and the class Ⅰ histone deacetylases were largely unknown. Here, we showed that the NP protein of IAV interacted with histone deacetylase 1 (HDAC1), which down-regulated the acetylation level of NP. Using mass spectrometry, we identified lysine 103(K103) as an acetylation site of the NP. Compared with wild-type protein, two K103 NP mutants, K103A and K103R, enhanced replication efficiency of the recombinant viruses in vitro. We further demonstrated that HDAC1 facilitated viral replication via two paths: promoting the nuclear retention of NP and inhibiting TBK1-IRF3 pathway. Our results lead to a new mechanism for regulating NP acetylation, indicating that HDAC1 may be a possible target for antiviral drugs.