AUTHOR=Rouet Romain , Jackson Katherine J. L. , Langley David B. , Christ Daniel 

TITLE=Next-Generation Sequencing of Antibody Display Repertoires

JOURNAL=Frontiers in Immunology

VOLUME=Volume 9 - 2018

YEAR=2018

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.00118

DOI=10.3389/fimmu.2018.00118

ISSN=1664-3224

ABSTRACT=In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage-, ribosome- and yeast-display, high affinity binders can be selected from diverse repertoires. Here we review strategies for the next generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment and affinity maturation.