AUTHOR=Fähnrich Anke , Klein Sebastian , Sergé Arnauld , Nyhoegen Christin , Kombrink Sabrina , Möller Steffen , Keller Karsten , Westermann Jürgen , Kalies Kathrin 

TITLE=CD154 Costimulation Shifts the Local T-Cell Receptor Repertoire Not Only During Thymic Selection but Also During Peripheral T-Dependent Humoral Immune Responses

JOURNAL=Frontiers in Immunology

VOLUME=Volume 9 - 2018

YEAR=2018

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.01019

DOI=10.3389/fimmu.2018.01019

ISSN=1664-3224

ABSTRACT=CD154 is a transmembrane cytokine expressed transiently on activated CD4 T cells upon T cell receptor stimulation that interacts with CD40 on antigen presenting cells. The signaling via CD154:CD40 is essential for B cell maturation and germinal center formation, but also for the final differentiation of CD4 T cells during T-dependent humoral immune responses. Recent data demonstrate that CD154 is critically involved in the selection of T cell clones during the negative selection process in the thymus. Whether CD154 signaling influences the T cell receptor repertoire during peripheral T-dependent humoral immune responses has not yet been elucidated. To find out, we used CD154 deficient mice and assessed the global T cell receptor β repertoire in T cell zones of spleens by high-throughput sequencing after induction of a Th2 response to the multiepitopic antigen sheep red blood cells. Qualitative and quantitative comparison of the splenic T-cell zone-specific T cell receptor β repertoires revealed that CD154 deficiency shifts the distribution of Vβ-Jβ genes after antigen exposure. This data led to the conclusion that costimulation via CD154:CD40 during the interaction of T cells with CD40 matured B cells contributes to the recruitment of T cell clones into the immune response and thereby shapes the peripheral T cell receptor repertoire.