AUTHOR=Pinto Ana Isabel , Smith Jennifer , Kissack Miriam R. , Hogg Karen G. , Green E. Allison 

TITLE=Thymic B Cell-Mediated Attack of Thymic Stroma Precedes Type 1 Diabetes Development

JOURNAL=Frontiers in Immunology

VOLUME=Volume 9 - 2018

YEAR=2018

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.01281

DOI=10.3389/fimmu.2018.01281

ISSN=1664-3224

ABSTRACT=Type 1 diabetes results from a co-ordinated autoimmune attack of insulin producing beta cells in the pancreas by the innate and adaptive immune systems, beta cell death being predominantly T cell-mediated. In addition to T cells, peripheral B cells are important in type 1 diabetes progression. The thymus of mice and man also contain B cells, and lately they have been linked to central tolerance of T cells. The role of thymic B cells in type 1 diabetes is undefined. Here we show there are abnormalities in the thymic B cell compartment prior to beta cell destruction and type 1 diabetes manifestation.
Using non-obese diabetic (NOD) mice, we document that preceding type 1 diabetes development, there is significant accumulation of thymic B cells-partly through in situ development- and the putative formation of ectopic germinal centres. In addition, in NOD mice we quantify thymic plasma cells and observe in situ binding of immunoglobulins to undefined antigens on a significant proportion of medullary thymic epithelial cells. In contrast, no ectopic germinal centres, or pronounced intrathymic autoantibodies are detectable in animals not genetically predisposed to developing type 1 diabetes. Binding of autoantibodies to thymic stroma correlates with apoptosis of medullary thymic epithelial cells, including insulin-expressing cells. In contrast, apoptosis of medullary thymic epithelial cells was decreased by 50% in B cell deficient NOD mice suggesting intrathymic autoantibodies may selectively target certain medullary thymic epithelial cells for destruction. Futhermore, we observe that these thymic B cell-associated events correlated with an increased prevalence of premature thymic emigration of T cells. 
Together our data suggests that the thymus may be a principal autoimmune target in type 1 diabetes and contributes to disease progression.