AUTHOR=Zhang Yuyu , Huo Wei , Sun Lidi , Wu Jie , Zhang Chengbin , Wang Huanhuan , Wang Bin , Wei Jinlong , Qu Chao , Cao Hongshi , Jiang Xin 

TITLE=Targeting miR-148b-5p Inhibits Immunity Microenvironment and Gastric Cancer Progression

JOURNAL=Frontiers in Immunology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.590447

DOI=10.3389/fimmu.2021.590447

ISSN=1664-3224

ABSTRACT=Background: MicroRNAs (miRNAs) have been discovered to dictate the development of various tumors. However, the roles of miRNAs in the progression of gastric cancer (GC) is still lacking.
Methods: Herein, by analyzing GC cell lines and patients samples, we observed that miR-148b-5p was significantly downregulated in GC. We also confirmed that miR-148b-5p overexpression significantly inhibited GC cells proliferation and invasion in vitro and in vivo. 
Results: Overexpression of miR-148b-5p not only reprogrammed the metabolic properties of GC but also regulated the immune microenvironment by shifting lymphocyte and myeloid populations. Mechanistically, ATPIF1, an important glycolysis-associated gene, was identified as a direct target of miR-148b-5p and mediated the effect of miR-148b-5p. Notably, low level of miR-148b-5p was significantly related with poor prognosis of GC patients. Importantly, the levels of miR-148b-5p significantly changed the sensitivity of GC cells to several anti-cancer drugs.  
Conclusions:Targeting miR-148b-5p inhibits immunity microenvironment and gastric cancer progression.