AUTHOR=Pérez-Sáez María José , Uffing Audrey , Leon Juliette , Murakami Naoka , Watanabe Andreia , Borges Thiago J. , Sabbisetti Venkata S. , Cureton Pamela , Kenyon Victoria , Keating Leigh , Yee Karen , Fernandes Satiro Carla Aline , Serena Gloria , Hildebrandt Friedhelm , Riella Cristian V. , Libermann Towia A. , Wang Minxian , Pascual Julio , Bonventre Joseph V. , Cravedi Paolo , Fasano Alessio , Riella Leonardo V. 

TITLE=Immunological Impact of a Gluten-Free Dairy-Free Diet in Children With Kidney Disease: A Feasibility Study

JOURNAL=Frontiers in Immunology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.624821

DOI=10.3389/fimmu.2021.624821

ISSN=1664-3224

ABSTRACT=Kidney disease affects 10% of the world population and is associated with increased mortality. Steroid-resistant nephrotic syndrome (SRNS) is a leading cause of end-stage kidney disease in children, often failing standard immunosuppression. Here, we report the results of a prospective study to investigate the immunological impact and safety of a gluten-free and dairy-free (GF/DF) diet in children with SRNS. The study was organized as a four-week summer camp implementing a strict GF/DF diet with prospective collection of blood, urine and stool in addition to WES of DNA of participants. Using flow cytometry, SOMAscan and microbiome metagenomics, we show that GF/DF diet had a major anti-inflammatory effect in all participants both at the protein and cellular level with 4-fold increase in Treg/Th17 ratio and the promotion of a favorable regulatory gut microbiota.  Overall, GF/DF can have a significant anti-inflammatory effect in children with SRNS and further trials are warranted to investigate this potential dietary intervention in children with SRNS.