AUTHOR=Wang Yunwei , Li Ming , Zeng Jiaoxia , Yang Yunshu , Li Zengshan , Hu Sijun , Yang Fangfang , Wang Na , Wang Wenlan , Tie Jun 

TITLE=MiR-585-5p impedes gastric cancer proliferation and metastasis by orchestrating the interactions among CREB1, MAPK1 and MITF

JOURNAL=Frontiers in Immunology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.1008195

DOI=10.3389/fimmu.2022.1008195

ISSN=1664-3224

ABSTRACT=MicroRNAs (miRNAs) were found to drive gastric cancer (GC) progression. However, the hidden pathogenesis that govern GC development remains uncertain. MiR-585-5p is highly downregulated within GC tissue, which was inversely linked to clinical prognosis. And we verified that miR-585-5p could effectively hinder proliferative / metastatic properties by synchronously targeting cyclic AMP-responsive element-binding protein 1 (CREB1), mitogen-activated protein kinase 1 (MAPK1) and microphthalmia-associated transcription factor (MITF). Furthermore, this investigation confirmed CREB1 activates MITF transcription and MAPK1 enhances MITF pro-cancerous activity via phosphorylation (Ser73), manifesting MITF acts as core target for miR-585-5p exerting effects. MiR-585-5p can not only inhibit MITF expression directly or indirectly, but also impede MITF activity in a MAPK1-dependent manner. In conclusion, this study unearthed miR-585-5p hampers GC proliferative / metastatic properties by orchestrating the interactions among CREB1, MAPK1 and MITF.