Carbon nanofibers (C139875-5G) were obtained from Aladdin (Shanghai, China). Fetal bovine serum (10099141C) and Iscove’s modified Dulbecco’s medium (IMDM; 12200036) were purchased from Gibco (Life Technologies, Gaithersburg, MD, USA). Trypsin–EDTA (MG0170) and penicillin–streptomycin (MG7989) were purchased from MacGene Biotechnology (Beijing, China). Nitric acid (225711), sulfuric acid (339741), sodium bicarbonate (S5761), thymidine (T1895), bathocuproine disulfonic acid disodium salt (B1125), l-cysteine (168149), and sodium pyruvate (P5280) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hypoxanthine (IH0490), dialysis membrane (YA1036) and Nuclear Extraction Kit (SN0020) were provided by Solarbio Life Science (Beijing, China). PrestoBlue cell viability reagents (A13261) were purchased from Invitrogen (Carlsbad, CA, USA). The annexin V-FITC Apoptosis Detection Kit (C1062L), Cell Cycle and Apoptosis Analysis Kit (C1052), Cell Counting Kit-8 (C0038), and one-step TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling) Apoptosis Assay Kit (C1088) were provided by Beyotime Biotechnology (Suzhou, China). The MMP assay kit (BB-3101), the intracellular Ca²⁺ assay kit (BB-48112), and the reactive oxygen assay kit (BB-47052) were provided by BestBio (Shanghai, China), while the ATP assay kit (A095-2-1) was provided by Jiancheng Bioengineering Institute (Nanjing, China). The HepG2 cell line was purchased from Cell Resource Center (Beijing, China). The T. brucei Lister strain 427 was maintained in our laboratory.

The GQDs were synthesized based on previous reports, with some modifications (34). Briefly, concentrated H₂SO₄ (60 ml) and HNO₃ (20 ml) were mixed with 0.3 g of carbon nanofibers. The mixture was ultrasonically stirred for 2 h and further stirred at 100°C for 24 h and the GQDs generated. After cooling, the solution containing the GQDs was diluted with 600 ml Milli-Q water. Sodium carbonate was used to adjust the pH to 8. The GQDs were further purified by dialysis with a molecular weight cutoff of 2,000 Da. The particle sizes of the GQDs were characterized using transmission electron microscopy (Hitachi HT7800, Tokyo, Japan). The fluorescence spectra of the GQDs were recorded using a fluorescence spectrophotometer (Hitachi F-7100, Tokyo, Japan).

The T. brucei Lister strain 427 was grown in an incubator at 37°C with 5% CO₂ in IMDM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (35). Cell culture dishes (25 cm²) and 96-well plates were used for the experiments.

T. brucei parasites (1 × 10⁶ cells/ml) were plated with a series of GQDs concentrations (0–400 μg/ml). After cultivation at 37°C and 5% CO₂ for 6 h in a humidified incubator, T. brucei parasites were harvested and washed three times with phosphate-buffered saline (PBS). The endocytosis of GQDs particles in T. brucei was examined in comparison with parasites that were not incubated with GQDs using flow cytometry (BD Biosciences, San Jose, CA, USA) and a laser scanning confocal microscope (TCS SP8; Leica, Wetzlar, Germany) with fluorescence excitation/emission (E _x/E _m) at 360/440 nm.

Inhibitors [Dynasore (36) and Pitstop2™ (37)] were used to investigate the clathrin-mediated endocytosis pathway. The parasites were grown in 96-well microplates (1 × 10⁶ cells/well) and pre-incubated for 30 min at 37°C with 10 and 20 μM Dynasore or 5 and 10 μM Pitstop2™. The parasites were maintained with 50 μg/ml GQDs for 3 h with 1 μM Dynasore or 0.5 μM Pitstop2™. To eliminate surface-adsorbed nanomaterials, T. brucei parasites were rinsed three times with PBS. The effect of Dynasore and Pitstop2™ on the endocytosis of GQDs by T. brucei was analyzed using flow cytometry and confocal microscopy.

The cell viability and proliferation after GQDs treatment were measured using the PrestoBlue™ (A13261; Invitrogen, Carlsbad, CA, USA) assay (38), which is a ready‐to‐use reagent for the rapid evaluation of the viability and proliferation of a wide range of cell types. In detail, viable cells continuously convert resazurin to highly fluorescent red resorufin, with increased overall fluorescence, and the cell viability can be detected using absorbance-based plate readers. T. brucei parasites (1 × 10⁶ cells/ml) were plated with varying concentrations of GQDs (0–400 μg/ml). After cultivation in a humidified incubator at 37°C with 5% CO₂ for 12 and 24 h, 20 µl of the PrestoBlue™ Cell Viability Reagent solution was added to the samples. Thereafter, an additional 10 min of incubation was carried out at 37°C. The cells were then examined using a microplate reader (PerkinElmer, Billerica, MA, USA) with E _x/E _m at 530/590 nm. T. brucei parasites that were not exposed to GQDs were used as controls.

Growth curves were determined by counting the parasites under a light microscope after being treated with GQDs at different time points. The abnormal nucleus and the kinetoplast morphology after GQDs treatment were calculated with a fluorescence microscope (39, 40). Parasite cells with one kinetoplast with one nucleus (1K1N), two kinetoplasts with one nucleus (2K1N), and two kinetoplasts with two nuclei (2K2N) were considered as normal cells, while parasites with 1K2N and xKyN (cells with multiple nuclei and kinetoplasts) were considered as abnormal cells.

Apoptosis was detected using the annexin V-FITC apoptosis detection kit (41). Briefly, T. brucei (1 × 10⁶ cells/ml) was plated with a series of GQDs concentrations (0–400 μg/ml). After cultivation in a humidified incubator at 37°C with 5% CO₂ for 12 and 24 h, apoptotic T. brucei cells, in comparison with parasites that were not exposed to GQDs, were examined according to the manufacturer’s instructions and analyzed using flow cytometry (BD Biosciences, San Jose, CA, USA).

Cell cycle assays were performed using Cell Cycle Analysis Kit (42). T. brucei (1 × 10⁶ cells/ml) was exposed to GQDs (0–400 μg/ml) for 12 and 24 h before being harvested and washed three times with PBS. The samples were fixed in 70% cold ethanol and incubated for 12 h at 4°C. After fixation, the cells were resuspended in cold PBS to remove ethanol. The cells were then stained with propidium iodide (PI) (1 mg/ml) containing 10 mg/ml RNase for 30 min at 37°C. The cell cycle was assessed using flow cytometry (BD Biosciences, San Jose, CA, USA). T. brucei parasites that were not exposed to GQDs were used as controls.

The intracellular reactive oxidative species (ROS) in T. brucei after treatment with GQDs were measured using a reactive oxygen detection kit (DHE-ROS) (43). T. brucei parasites (1 × 10⁶ cells/ml) were exposed to GQDs (0–400 μg/ml) for 12 and 24 h, and the samples were treated according to the manufacturer’s instructions and examined by flow cytometry at 488 nm excitation and 610 nm emission wavelengths (BD Biosciences, San Jose, CA, USA). T. brucei parasites cultivated simultaneously without exposure to GQDs were used as controls.

The ATP levels in T. brucei after treatment with GQDs were measured using a firefly luciferase assay, which catalyzes the generation of luciferin in the presence of ATP (44). Briefly, T. brucei parasites (1 × 10⁶ cells/ml) were cultivated with varying doses of GQDs (0–400 μg/ml) for 12 and 24 h before the they were harvested. After lysis, the chemiluminescence of the supernatant was monitored using a microplate reader (PerkinElmer, Billerica, MA, USA). T. brucei parasites not incubated with GQDs were used as controls.

T. brucei parasites (1 × 10⁶ cells/ml) were exposed to GQDs (0–400 μg/ml) for 12 and 24 h. Pathophysiological changes in the mitochondrial membrane potential (MMP) were measured using a JC-10 fluorescent probe, which accumulates in the matrix of mitochondria at high MMP and forms polymers emitting red fluorescence. On the contrary, at low MMP, JC-10 forms monomers emitting green fluorescence in the matrix of the mitochondria. The fluorescence of JC-10 aggregates and JC-10 monomers was detected by flow cytometry with an excitation wavelength of 488 nm and emission wavelengths of 570 and 520 nm, respectively (45). T. brucei parasites that were not exposed to GQDs were used as controls.

T. brucei DNA fragmentation after treatment with GQDs was analyzed using a TUNEL assay, which uses terminal deoxynucleotidyl transferase to add fluorescently labeled dUTP to the 3′-OH ends of the DNA fragments, which resulted from the apoptotic process (46). T. brucei parasites (1 × 10⁶ cells/ml) were cultivated with varying doses of GQDs (0–400 μg/ml) for 12 and 24 h before the they were harvested. Apoptotic cells with DNA fragmentation were detected and analyzed using flow cytometry with E _x/E _m at 475/520 nm. T. brucei parasites that were not exposed to GQDs were used as controls.

Cytosolic Ca²⁺ concentrations were measured using BBcellProne^® F03 (BB-48112; BestBio Technical, Beijing, China) according to the manufacturer’s instructions (47). T. brucei parasites (1 × 10⁶ cells/ml) were cultivated using a variety of GQDs doses (0–400 μg/ml) for 12 and 24 h. Thereafter, the cells were collected and washed three times in PBS. Subsequently, the parasites were treated with the BBcellProne^® F03 staining working solution at 37°C for 30 min, washed three times with PBS, and cultivated for a further 30 min in the culture medium. The fluorescence intensity of the parasites was obtained by obtaining the emission at 488 nm using flow cytometry (BD Biosciences, San Jose, CA, USA) and further examining with a laser scanning confocal microscope (TCS SP8; Leica, Wetzlar, Germany). T. brucei parasites not incubated with GQDs were used as controls.

The nuclei of T. brucei cells were isolated using a nuclear extraction kit (SN0020; Solarbio, Beijing, China) following the manufacturer’s instructions. The 1× SDS-PAGE loading buffer was mixed with the samples before being heated at 100°C for 5 min. Afterward, the proteins were separated by 12% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes. Before co-incubation with rabbit anti-tSNAP42 immunoglobulin G (IgG) (1:1,000), rabbit anti-endonuclease G (Endo G) IgG (1:1,000), and rabbit anti-histone 3 IgG (1:1,000), the membranes were blocked with 5% skim milk at 37°C for 1 h. Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (SE134; Solarbio, Beijing, China) was used as the secondary antibody. The nuclear proteins of T. brucei parasites not exposed to GQDs were used as controls.

A glutaraldehyde solution (2.5%) was used to fix T. brucei parasites treated with GQDs. After washing with PBS, the cells were blocked with 5% (w/v) skim milk. Rabbit anti-tSNAP42 IgG and rabbit anti-Endo G IgG were added to the cells. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Finally, the fluorescence intensity of the parasites was determined by measuring the emission at 488 nm using flow cytometry (BD Biosciences, San Jose, CA, USA). T. brucei parasites that were not exposed to GQDs were used as controls.

After exposure to GQDs (50 μg/ml) for 24 h, T. brucei parasites (1 × 10⁷ cells/ml) were dissolved in TRIzol reagent for the extraction of total RNA. The enrichment of messenger RNA (mRNA) was carried out with Oligo(dT) beads and the Ribo-Zero™ Magnetic Kit (MRZH11124; Epicenter Biotechnologies, Madison, WI, USA). RNA sequencing (RNA-seq) was performed by Gene Denovo Biotechnology (Guangzhou, China) using Illumina HiSeq2500.

Differential transcription analysis of mRNA was conducted using DESeq2 software (48) and edgeR (49) between the sequences of GQD-treated and untreated parasites. A false discovery rate (FDR) ≤0.05 and fold change ≥1.5 were used as the thresholds for differentially expressed genes (DEGs). Proteins encoded by DEGs were subjected to Gene Ontology (GO) (50) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses (51). Significantly enriched GO terms or pathways were defined as FDR ≤ 0.05.

After exposure to GQDs (50 μg/ml) for 24 h, T. brucei parasites (1 × 10⁷ cells/ml) were dissolved in TRIzol reagent for the extraction of total RNA. SYBR^® Premix Ex Taq™ (RR820A; TaKaRa, Shiga, Japan) was used for real-time PCR. The specific primers corresponding to the genes coding for both trypanothione synthetase and TryR are listed in Supplementary Table S1 . The gene transcription level was determined using the 2^−ΔCt method, which compares the quantity of the target RNA to that of the gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Tb927.6.4280), which was used as an internal control (52).

Thin smears of T. brucei were fixed in cold methanol for 10 min before blocking in 5% skim milk. The mouse anti-TryR antibody was used as the primary antibody at a dilution of 1:50. Alexa Fluor 594-conjugated goat anti-mouse IgG (SE134; Solarbio, Beijing, China) was used as the secondary antibody at a dilution of 1:600. The nuclei of the parasites were stained with DAPI. T. brucei parasites that were not exposed to GQDs were used as controls. Serum from an unimmunized mouse and PBS buffer without antibodies were used as the negative and blank controls, respectively. High-resolution images were captured using a confocal laser scanning microscope (SP8; Leica, Wetzlar, Germany).

The total lysate of T. brucei that received GQDs treatment was mixed with the SDS-PAGE loading buffer, and the proteins were separated using 12% SDS-PAGE. Subsequently, the proteins were transferred onto PVDF membranes. Before co-incubation with mouse anti-TryR IgG (1:1,000), the membranes were blocked with 5% skim milk at 37°C for 1 h. HRP-conjugated goat anti-rabbit IgG (SE134; Solarbio, Beijing, China) was used as the secondary antibody. Signals obtained with rabbit anti-GAPDH IgG were used for normalization.

The gene coding for the TryR of T. brucei (Tb927.10.10390) was amplified from parasite complementary DNA (cDNA) and cloned into the expression vector pET-28a in Escherichia coli BL21 (DE3) cells. The His-tagged recombinant protein (TryR-His) was purified using NiNTA agarose (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions.

The interaction kinetics between the GQDs and TryR-His were studied using an Octet K2 device (ForteBio, Menlo Park, CA, USA). Firstly, the Ni-NTA biosensors were coated with TryR-His (50 μg/ml). GQDs at a series of concentrations were then loaded onto the Ni-NTA biosensors. Subsequently, the binding affinities of the GQDs (diluted from 800 to 12.5 nM) with TryR-His were determined. The association and dissociation between GQDs and TryR-His were monitored for 5 min, and Octet Data Analysis software (version 7.0; ForteBio, Menlo Park, CA, USA) (53) was used to calculate the affinities and kinetic parameters.

All experiments were performed in triplicate. Student’s t-test was used to analyze the data. All values are presented as the mean ± SD of triplicate measurements. *p < 0.05 was considered significant, **p < 0.01 considered highly significant, and ***p < 0.001 was considered very highly significant.

The synthesized GQDs were morphologically characterized using a transmission electron microscope (TEM) ( Figure 1A ). The size distribution of the GQDs was 4.23 ± 0.94 nm (n = 100) ( Supplementary Figure S1 ). The GQDs were scanned using a fluorescence spectrophotometer to obtain the fluorescence spectra ( Figure 1B ). Based on the fluorescence spectrum, an excitation wavelength of 360 nm and an emission wavelength of 440 nm were chosen to analyze the endocytosis of GQDs by T. brucei.

The entry of GQDs into T. brucei was observed using a laser scanning confocal microscope. After co-incubation with T. brucei cells, a wide distribution of GQDs in the cytoplasm of the parasites was observed ( Figure 1C ), and the quantity of intracellular GQDs was concentration-dependent ( Figure 1D ). Considering that trypanosome parasites utilize the clathrin-mediated endocytosis pathway (54), two well-known clathrin inhibitors (Pitstop2™ and Dynasore) were selected for the analysis of the transportation of GQDs into T. brucei. The uptake of GQDs by T. brucei was considerably reduced in a concentration-dependent manner when these inhibitors were used ( Figure 1E ). Prior to the experiment, the toxicity of the two inhibitors to parasites was determined using a Cell Counting Kit-8 assay. Both Pitstop2™ and Dynasore did not show cytotoxicity to parasites at the applied concentrations ( Supplementary Figure S2 ). This finding suggests that T. brucei endocytosed GQDs via a clathrin-dependent pathway.

The cytotoxicity of GQDs is believed to be closely associated with their size, charge, the biological activity (functional groups) of the outer layer, oxidation, photolysis, and mechanical stability (55, 56). Although studies have demonstrated that ultra-small nanomaterials are less cytotoxic than large-sized nanomaterials (>100 nm), the ultra-small GQDs we generated displayed outstanding cytotoxicity to T. brucei. Treatment with GQDs decreased the cell proliferation based on the proliferation curve ( Figure 2A ) and the arrest of cell development ( Supplementary Figure S3 ) of T. brucei, and the half maximal effective concentration (EC₅₀) for GQDs at 24 h was 27.16 μg/ml. Based on the assessment of HepG2 cells ( Supplementary Figure S4 ) and on previous reports (57), the doses of the GQDs explored in this study were not toxic to mammalian cells. The annexin V-FITC/PI apoptosis assay (58) was used to assess the apoptosis of T. brucei cells following treatment with GQDs. GQDs mostly promoted advanced apoptosis in T. brucei ( Figure 2B ).

Although the morphological characteristics of T. brucei were not altered significantly ( Figure 2C and Supplementary Figure S5 , SEM images), a series of classical cell apoptosis features with subcellular morphological defects were observed after exposure to GQDs, similar to the cytopathological alterations caused by other nanomaterials (59). The pathophysiological changes in T. brucei subcellular organelles ( Figure 2C , TEM images) were dramatic following treatment with GQDs, including mitochondrial (M) morphological alterations and a swollen Golgi apparatus (G). Additionally, the perinuclear space (PS) was severely swollen after exposure to GQDs. Dilatation of the endoplasmic reticulum (ER) was also observed (60).

An increase in ROS level is a key feature of nanoparticle-induced apoptosis in mammalian cells. The administration of GQDs led to a tremendous increase in the intracellular ROS levels, which was accompanied by a progressive reduction in the MMP (ΔΨ _m) in a dose-dependent manner ( Figures 3A, B ).

The intracellular accumulation of free radicals and the increased Ca²⁺ levels can lead to MMP (ΔΨ _m) depression, further leading to cell apoptosis. We subsequently investigated alterations in the concentrations of Ca²⁺ in T. brucei after incubation with GQDs. Treatment with GQDs resulted in intracellular Ca²⁺ accumulation in T. brucei ( Figure 3C ). Furthermore, defects in the ATP generation have been regarded as a consequence of mitochondrial dysfunction and apoptosis. In this study, GQDs were also found to effectively suppress the ATP generation in T. brucei ( Figure 3D ), which was congruent with the loss of MMP and the elevation of the concentrations of intracellular ROS and Ca²⁺. Moreover, treatment with GQDs resulted in developmental arrest in the sub-G0/G1 phase of T. brucei cells and a concomitant decline in G2/M phase cells ( Figure 3E ), which is typically caused by DNA fragmentation. Considerable DNA fragmentation in T. brucei was also observed after 24 h of exposure to GQDs ( Figure 3F ). Considering that GQDs did not exhibit cytotoxic effects on the host cells, these data indicate that GQDs exerted specific biological inhibition on trypanosomal parasites.

In T. brucei, prolonged ER stress induces an apoptotic mechanism called splice leader RNA silencing (SLS), which resembles apoptosis mediated by caspases observed in eukaryotic organisms (61). The hallmark of SLS is the shut-off of the transcription of the splice leader RNA (SL RNA) and the massive accumulation of tSNAP42, a specific transcription factor that binds the promoter of SL RNA (62). In this study, exposure to GQDs caused a significant elevation of tSNAP42 in the cell nuclear fraction after prolonged ER stress, as revealed by Western blotting ( Figures 4A, B ). Although trypanosomatids have an apoptotic mechanism independent of caspases, Endo G, a well-described nuclease released from the mitochondria and its role in DNA fragmentation following trafficking to the nucleus have been documented (63). Therefore, we used Western blotting to investigate organelle fractionation in T. brucei following exposure to GQDs and observed considerable accumulation of Endo G in the nuclear fractions ( Figures 4C, D ). Flow cytometry studies also revealed that the intracellular expression levels of tSNAP42 and Endo G were remarkably increased following exposure to GQDs, suggesting that the mitochondrial pathway and ER stress were indeed pivotal in the apoptosis of T. brucei induced by GQDs ( Figures 4E, F ).

To further investigate the molecular mechanisms of apoptosis in T. brucei induced by treatment with GQDs, parasite RNAs were sequenced after treatment (50 µg/ml) for 24 h. Among the 10,277 transcripts identified, 783 were found to be differentially transcribed, some of which were linked to mitochondrial function, oxidative stress, energy metabolism, glycometabolism, and lipid metabolism ( Figures 5A, B ). The most upregulated gene was a gene coding for the variant surface glycoprotein (6.7, Tb927.3.5900). The expression levels of the GPEET2 procyclin precursor (3.1, Tb927.6.510), EP1 procyclin precursor (2.4, Tb927.10.10260), and the protein encoded by the expression site-associated gene 1 (ESAG 1) (3.5, Tb927.3.2520) were also highly upregulated. In addition, the upregulation of iron/ascorbate oxidoreductase (2.1, Tb927.2.6270) was closely related to the redox regulatory system. The gene with the most pronounced fold downregulation was adenylate cyclase (−4.4, Tb927.11.1490), which was most likely due to the reduction in ATP production ( Dataset S1 ), indicating elevated response to the GQDs treatment.

We used GO and KEGG enrichment analyses with a corrected p-value of 0.05 as the threshold to acquire more thorough insights into the functions of the DEGs. Protein function, chromosome associates, peroxisomes, and pyruvate metabolism were among the top 10 enriched terms ( Figures 5C, D ). Furthermore, genes involved in glycerolipid metabolism, which is closely related to lipid peroxidation, were significantly enriched. This also indicates that the antioxidant capacity of the parasites may have been profoundly impaired by GQDs treatment. Moreover, the genes involved in the glycolysis/gluconeogenesis and pentose phosphate pathways showed a significant enrichment, indicating that T. brucei may perform a correspondent adjustment in the energy metabolism under GQDs treatment ( Figure 5C ).

Trypanosomal parasites do not contain the conventional disulfide reductases (glutathione reductase and thioredoxin reductase) seen in higher eukaryotes and have developed a distinctive redox system relying on trypanothione, which is considered to be a protective mechanism that maintains intracellular redox homeostasis (64). Quantitative reverse transcription PCR (qRT-PCR) ( Figure 5E ) and RNA-seq ( Supplementary Figure S12A ) revealed the enhanced expression of trypanothione synthetase in response to GQDs treatment, indicating that GQDs induced oxidative stress in parasites. However, the expression of TryR was slightly suppressed in the transcriptomic analysis ( Supplementary Figures S12B and S13 ), which was different from the result of qRT-PCR ( Figure 5F ). This was likely caused by intrinsic deficiency of the RNA-seq approach (65–67). Multiple qRT-PCR experiments showed that the expression of TryR was upregulated after treatment with GQDs.

Previous studies have reported that nanomaterials can be efficiently endocytosed, resulting in increased intracellular reactive oxygen levels through interactions with intracellular proteins and DNAs (55). Nanomaterials have also been shown to affect the secondary structure of proteins, which may cause changes in their functions (68). TryR is a pivotal enzyme in the response to oxidative stress in trypanosomal parasites, and its expression and activity have a substantial impact on the antioxidant capability. Cell physiological analysis revealed that the redox capability was impeded by GQDs treatment. To confirm this, a total lysate of T. brucei containing TryR was obtained for the test of antioxidant activity with trypanothione as the substrate. The antioxidant capacity of the lysate from parasites pre-exposed to GQDs was significantly lower than that of untreated parasites ( Figure 6A ). Recombinant TryR was obtained and confirmed using Western blotting analysis ( Figure 6B ). GQDs inhibited the activity of recombinant TryR (TryR-His) in a dose-dependent manner ( Figure 6C ). The affinity of GQDs to TryR-His was analyzed using the Octet K2 system, which tracked the binding (k _on) and dissociation k _dis) rates between the two molecules. The dissociation constant (K _d) for the binding between TryR-His and GQDs was 5.04E−09. This suggests that GQDs have a high affinity for TryR ( Figure 6D and Supplementary Table S2 ), which explains their dramatic antiparasitic effects. Furthermore, immunofluorescence indicated that the intracellular distribution of TryR was not affected by treatment with GQDs ( Figure 6E ). GQDs were not observed in the DAPI channel of the second row because they were below the detection threshold for this exposure time. Western blotting analysis showed that the expression of TryR also increased in response to GQDs treatment ( Figures 6F, G ). These data indicate that the activity of TryR was inhibited after GQDs treatment, which ultimately resulted in the constant accumulation of ROS in the parasites and the onset of apoptosis ( Figures 3A and 7 ).