AUTHOR=Seaver Kyle , Kourko Olena , Gee Katrina , Greer Peter A. , Basta Sameh 

TITLE=IL-27 Improves Prophylactic Protection Provided by a Dead Tumor Cell Vaccine in a Mouse Melanoma Model

JOURNAL=Frontiers in Immunology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.884827

DOI=10.3389/fimmu.2022.884827

ISSN=1664-3224

ABSTRACT=The protocol used to induce cell death for generating vaccines from whole tumour cells is a critical consideration that impacts vaccine efficacy. Here we compared how different protocols used to induce cell death impacted protection provided by a prophylactic whole tumour cell vaccine in a mouse melanoma model. We found that melanoma cells exposed to -irradiation or lysis combined with UV-irradiation (LyUV) provided better protection against tumour challenge than lysis only or cells exposed to UV-irradiation. Furthermore, we found that the immunoregulatory cytokine, IL-27 enhanced protection against tumour growth in a dose-dependent manner when combined with either LyUV or -irradiated whole tumour cell vaccine preparations. Taken together, this data supports the use of LyUV as a potential protocol for developing whole tumour cell prophylactic cancer vaccines. We also showed that IL-27 can be used at low doses as a potent adjuvant in combination with LyUV or -irradiation treated cancer cells to improve the protection provided by a prophylactic cancer vaccine in a mouse melanoma model.