AUTHOR=Hong Junping , Wei Dongmei , Zhong Ling , Wu Qian , Chen Kaiyun , Zhang Wanlin , Yang Yanbo , Chen Junyu , Xia Ningshao , Zhang Xiao , Chen Yixin TITLE=Glycoprotein B Antibodies Completely Neutralize EBV Infection of B Cells JOURNAL=Frontiers in Immunology VOLUME=Volume 13 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.920467 DOI=10.3389/fimmu.2022.920467 ISSN=1664-3224 ABSTRACT=
The Epstein–Barr virus (EBV) is the first reported oncogenic herpesvirus that establishes persistent infection in B lymphocytes in 95% of adults worldwide. Glycoprotein B (gB) plays a predominant role in the fusion of the viral envelope with the host cell membrane. Hence, it is of great significance to isolate gB-specific fusion-inhibiting neutralizing antibodies (NAbs). AMMO5 is the only gB NAb but fails to antagonize B-cell infection. It is essential to isolate potent NAbs that can completely block EBV infection of B cells. Using hybridoma technology and neutralization assay, we isolate two gB NAbs 8A9 and 8C12 that are capable of completely neutralizing B-cell infection